Endocrine Abstracts

Metabolic reprogramming resulting in significant alterations of the energy metabolism is a hallmark of prostate cancer (PCa). In this study we performed a comprehensive metabolic analysis of various human (LNCaP, DuCaP, PC-3, Du145) and murine PCa cell lines differing in the expression of the tumor suppressor phosphatase and tensin homolog (PTEN). In line with previous studies we found that PTEN- PCa cells (LNCaP, PC-3) had a higher glycolytic activity than PTEN<sup...

The use of more tissue-mimetic 3-dimensional (3D) cell culture models for in vitro drug screening has significantly increased in the past with the expectation to overcome with over-interpretation of drug effects obtained with conventional 2D cultures. In this study, we characterized 3D prostate cancer (PCa) organoids where PCa epithelial cells (LNCaP, DuCaP, LAPC4) were cultured alone or with PCa-associated fibroblasts (CAFs) in 96 well hanging drop plates in order to...